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IF-FISH with 53BP1 (Abcam, ab175933), PML (MilliporeSigma, 05-718), or ACA (anti-centromere antibody, Antibodies, Davis, CA, 15-235) was performed as described previously , with minor.. The primary antibody was goat polyclonal, ab87639 (Abcam, Cambridge, MA, USA). The secondary was an IRDye 680 Donkey anti-goat antibody (926-68074). Western detection methods were followed..
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